Improvement of aminolevulinic acid (ALA)-mediated photodynamic diagnosis using n-propyl gallate

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• 作者：Tomohisa Hirano^a ; Yuichiro Hagiya^a ; Hideo Fukuhara^b ; Keiji Inoue^b ; Taro Shuin^b ; Kentaro Matsumoto^a ; Katsushi Inoue^c ; Tohru Tanaka^c ; Ichiro Okura^a ; Shun-ichiro Ogura ; PhD^a ; ^{sogura@bio.titech.ac.jp}
• 关键词：Aminolevulinic acid ; n-Propyl gallate ; Protoporphyrin IX ; Photodynamic diagnosis
• 刊名：Photodiagnosis and Photodynamic Therapy
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：10
• 期：1
• 页码：28-32
• 全文大小：793 K

文摘

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Summary

Background

Photodynamic diagnosis (PDD) using aminolevulinic acid (ALA) is widely used in clinical fields. In PDD, protoporphyrin IX (PpIX) is generated from ALA in tumors, allowing the detection of the tumors by PpIX fluorescence. However, it is well known that PpIX is bleached by light irradiation (photobleaching) resulting in reduced PDD efficiency. In this study, n-propyl gallate (NPG) was investigated as an enhancer of PDD efficiency.

Methods

Tumor cells were incubated with NPG after treatment with ALA, and reactive oxygen species and PpIX fluorescence were measured.

Results

The antioxidant NPG suppressed the production of reactive oxygen species from light-irradiated porphyrins and ameliorated photobleaching of PpIX generated from ALA in vitro and in vivo.

Conclusion

Incubation with NPG decreased the production of reactive oxygen species from PpIX and suppressed PpIX photobleaching. These results indicate that the antioxidant NPG may significantly improve PDD efficiency.