gp85 protein vaccine adjuvanted with silica nanoparticles against ALV-J in chickens

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• 作者：Jia Cheng^a ; ¹ ; Shiyong Wen^b ; ^c ; ¹ ; Shenghua Wang^a ; Pan Hao^a ; Ziqiang Cheng^a ; Yongxia Liu^a ; Peng Zhao^d ; ^{zhaopeng@sdau.edu.cn} ; Jianzhu Liu^a ; ^{Liujz@sdau.edu.cn}
• 关键词：Silica nanoparticles ; gp85 protein ; ALV-J ; Chicken
• 刊名：Vaccine
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：35
• 期：2
• 页码：293-298
• 全文大小：883 K
• 卷排序：35

文摘

This study focused on the effect of silica nanoparticles as adjuvant for vaccine applications comprised of gp85, a dominating structural protein of J Subgroup Avian Leukosis Virus (ALV-J), and which was evaluated by comparing with the responsiveness induced by that emulsified in Freund adjuvant. Thirty-six chickens were inoculated twice with gp85 adjuvanted with the silica nanoparticles or Freund’s adjuvant at the 2nd and 3rd week old. Two weeks later, the inoculated chickens were challenged with a 102.2 50% tissue culture infective dose (TCID50) of ALV-J. The blood samples were collected weekly to detect the serum antibodies and viremia. Results showed that positive serum antibodies (S/P value > 0.6) against gp85 emerged at the third week in the inoculated chickens, while the antibodies level persisted longer in silica nanoparticles adjuvanted-group to Freund’s adjuvanted-group. Furthermore, viremia in silica nanoparticles adjuvanted-group was recovered more quickly compared with Freund’s adjuvanted-group. Hence our study revealed that silica nanoparticles can effectively improve the protection of gp85 vaccine against ALV-J and present a better performance than Freund’s adjuvant.