Where Do We Stand with Super-Resolution Optical Microscopy?
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- 作者：Karin Nienhaus1 ; G. Ulrich Nienhaus1 ; 2 ; 3 ; 4 ; uli@uiuc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：PALM ; photoactivated localization microscopy ; STED ; stimulated emission depletion ; FCS ; fluorescence correlation spectroscopy ; RICS ; raster image correlation spectroscopy ; FP ; fluorescent protein ; GFP ; green fluorescent protein ; SPT ; single-particle tracking ; RESOLFT ; reversible saturable optical fluorescence transitions ; SIM ; structured illumination microscopy ; STORM ; stochastic optical reconstruction microscopy ; SPIM ; selective plane illumination microscopy ; FFS ; fluorescence fluctuation spect
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：428
- 期：2
- 页码：308-322
- 全文大小：1610 K
文摘
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Fluorescence microscopy enables imaging of specific biomolecules in live specimens under near-physiological conditions.
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Key approaches to achieve super-resolution in fluorescence microscopy are discussed.
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Fluorescence fluctuation spectroscopy techniques allow quantification of fast biomolecular dynamics.
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How to choose the optimal fluorescence marker for a specific imaging technique.
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Where are we going with advanced optical imaging?