Paramagnetic epoxy-functionalized mesostructured cellular foams with an open pore system for immobilization of penicillin G acylase

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• 作者：Ling Yang ; Zhenyuan Gao ; Yanglong Guo ; ^{ylguo@ecust.edu.cn" class="auth_mail} ; Wangcheng Zhan ; Yun Guo ; Yunsong Wang ; Guanzhong Lu ; ^{gzhlu@ecust.edu.cn" class="auth_mail}
• 关键词：Mesostructured cellular foams ; Paramagnetic ; Epoxy groups ; Penicillin G acylase ; Immobilization
• 刊名：Microporous and Mesoporous Materials
• 出版年：15 May, 2014
• 年：2014
• 卷：190
• 期：Complete
• 页码：17-25
• 全文大小：1772 K

文摘

Paramagnetic epoxy-functionalized mesostructured cellular foams (PEMCFs), prepared by reacting the epoxy groups grafted on the outer surface of epoxy-functionalized MCFs with the amino groups of l-cysteine modified on the surface of Fe₃O₄ nanoparticles, were characterized by SAXS, powder XRD, nitrogen sorption, TEM, FT-IR, TG, ICP-AES and magnetic susceptibility measurements, and investigated as the support for immobilization of penicillin G acylase (PGA). The results show that paramagnetic Fe₃O₄ nanoparticles were successfully grafted covalently on the outer surface of PEMCFs due to the larger particle size of Fe₃O₄ nanoparticles, and PGA was successfully immobilized covalently on the inner surface of PEMCFs. PGA immobilized on PEMCFs shows the highest initial activity and operational stability among those ever reported in the literatures, which had the initial activity of 8800 U g^鈭? and retained 94.5% of its initial activity after recycled for 10 times. PGA immobilized on PEMCFs can be easily recycled by the aid of an external magnetic field in order to replace the tedious separation of high-speed centrifugation.