Evaluation of kinetic parameters of immobilized penicillin G acylase subject to an inactivation and reactivation process
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- 作者：Oscar Romero ; Erick Araya ; André ; s Illanes ; Lorena Wilson ; lwilson@ucv.cl" class="auth_mail
- 关键词：Inactivaction ; Kinetic parameters ; Reactivation ; Penicillin acylase
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：June 2014
- 年：2014
- 卷：104
- 期：Complete
- 页码：70-74
- 全文大小：576 K
文摘
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Immobilized PGA was inactivated by organic solvent and reactivated by two strategies.
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Apparent and intrinsic kinetic parameters were calculated in three biocatalyst states.
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Catalyst inactivation and reactivation significantly impacted the kinetic parameters.
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Organic solvent caused conformation changes that were not restored by reactivation.