Pretreatment-free detection of diazepam in beverages based on a thermometric biosensor
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- 作者：Ning XuAuthor Vitae ; Jialei BaiAuthor Vitae ; Yuan PengAuthor Vitae ; Zhiwei QieAuthor Vitae ; Zengshan LiuAuthor Vitae ; Hongzhi TangAuthor Vitae ; Chao LiuAuthor Vitae ; Zhixian Gao ; gaozhx@163.comAuthor Vitae ; Baoan Ning ; ningba@163.comAuthor Vitae
- 关键词：Diazepam ; Beverages ; Immunoassay ; Enzyme thermistor (ET) ; Pretreatment-free
- 刊名：Sensors and Actuators B: Chemical
- 出版年：2017
- 出版时间：31 March 2017
- 年：2017
- 卷：241
- 期：Complete
- 页码：504-512
- 全文大小：1732 K
- 卷排序：241
文摘
A FIA-TELISA method for the pretreatment-free detection of diazepam (DZP) in real samples was constructed based on a direct competition. This method had good specificity and repeatability, and possessed high consistence with HPLC method. Our studies provided a promising assay model which was capable of continuously monitoring analytes without elaborate extraction procedures to correct the matrix effect of the beverages.