Systematic evaluation of monoclonal antibodies and immunoassays for the detection of Interferon-γ and Interleukin-2 in old and new world non-human primates

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• 作者：Ankie Hö ; glind^a ; Irene Areströ ; m^a ; Cecilia Ehrnfelt^a ; Khosro Masjedi^a ; Bartek Zuber^b ; Luis Giavedoni^c ; Niklas Ahlborg^a ; ^d ; ^{niklas@mabtech.com}
• 关键词：AIDS ; acquired immunodeficiency virus ; AGM ; African Green monkey ; AOT ; Ma's night monkey ; BAB ; Baboon ; CMV ; Cytomegalo virus ; CYN ; cynomolgus macaque ; FCS ; Fetal calf serum ; GFP ; green fluorescent protein ; HUM ; Human ; IFN ; Interferon ; IL ; Interleukin ; mAb ; Monoclonal antibody ; MAR ; common marmoset ; NHP ; Non-human primates ; PBMC ; Peripheral blood mononuclear cells ; PBS ; Phosphate-buffered saline ; PTM ; pigtail macaque ; RHE ; Rhesus macaque ; RT ; Room temperature ; SAI ; Squirrel monkey ; SIV ; Simian im
• 刊名：Journal of Immunological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：441
• 期：Complete
• 页码：39-48
• 全文大小：1592 K
• 卷排序：441

文摘

Panels of mAbs to human IFN-γ and IL-2 were assessed for cross-reactivity with NHP. NHP included both Old and New World species. Pan-reactive mAbs recognizing cytokines from all NHP species were identified. Capture and detection mAb pairs reactive with all NHP species were developed. Functionality of mAbs was verified in ELISA, ELISpot, FluoroSpot and flow cytometry.