Reactive gamma-ketoaldehydes as novel activators of hepatic stellate cells in vitro

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• 作者：Lisa Longato^a ; Fausto Andreola^b ; Sean S. Davies^c ; Jackson L. Roberts^c ; Giuseppe Fusai^d ; Massimo Pinzani^a ; Kevin Moore^a ; ¹ ; Krista Rombouts^a ; ¹ ; ^{k.rombouts@ucl.ac.uk}
• 关键词：15-E2-IsoLG ; isolevuglandin E2 ; 4-HNE ; 4-hydroxy-2-nonenal ; α-SMA ; alpha Smooth Muscle Actin ; ANOVA ; analysis of variance ; ATF4 ; Activating transcription factor 4 ; BiP ; Immunoglobulin heavy chain binding protein ; BrdU ; 5-Bromo-2-Deoxyuridine ; carboxy-DCF ; (5(6)-Carboxy-2&prime ; 7&prime ; -dichlorofluorescein ; carboxy-H2DCFDA ; 2&prime ; 7&prime ; -dichlorodihydrofluorescein diacetate ; CHOP ; CCAAT-enhancer-binding protein homologous protein ; CCL2 ; Chemokine (C-C motif) ligand 2 ; CM ; Complete Medium ; C
• 刊名：Free Radical Biology and Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：102
• 期：Complete
• 页码：162-173
• 全文大小：2502 K
• 卷排序：102

文摘

Isolevuglandins (IsoLG) are described as novel activators of hepatic stellate cells. IsoLG are biologically active at concentrations as low as 500 pM. IsoLG promote activation of stress signaling pathways and redox changes. IsoLG induce a pro-inflammatory effect, in part mediated by autophagy activation. Autophagy may reduce toxicity from IsoLG adduction to cellular proteins.