A simple, low-cost, and reusable optofluidic platform was developed for direct and ultrasensitive immunoassay of AFM1.
Effect of organic solvents on interaction of antibody-antigen in heterogeneous and homogeneous solutions was evaluated.
AFM1 can be directly quantified by the proposed platform even if the samples contain a certain organic solvent.
This method provides the LOD of 5 ng/L for AFM1 in dairy products.
This method provides a tool for the rapid, accurate, and reliable detection of AFM1 with a simple sample pretreatment.