CoVaccine HT™ adjuvant is superior to Freund's adjuvants in eliciting antibodies against the endogenous alarmin HMGB1

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• 作者：Nerissa Lakhan<sup>asup><sup> ; sup><sup>bsup> ; Natalie E. Stevens<sup>asup> ; Kerrilyn R. Diener<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>kerrilyn.diener@adelaide.edu.ausup> ; John D. Hayball<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>john.hayball@unisa.edu.ausup>
• 关键词：CV ; CoVaccineHT ; FA ; Freund's adjuvant ; CFA ; Complete Freund's adjuvant ; IFA ; Incomplete Freund's adjuvant ; SFASE ; sucrose fatty acid sulphate ester ; HMGB1 ; high mobility group box-1 ; Ab ; antibody ; Abs ; antibodies
• 刊名：Journal of Immunological Methods
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：439
• 期：Complete
• 页码：37-43
• 全文大小：569 K
• 卷排序：439

文摘

Adjuvants are used to enhance the immune response against specific antigens for the production of antibodies, with the choice of adjuvant most critical for poorly immunogenic and self-antigens. This study quantitatively and qualitatively evaluated CoVaccine HT™ and Freund's adjuvants for eliciting therapeutic ovine polyclonal antibodies targeting the endogenous alarmin, high mobility group box-1 (HMGB1). Sheep were immunised with HMGB1 protein in CoVaccine HT™ or Freund's adjuvants, with injection site reactions and antibody titres periodically assessed. The binding affinity of antibodies for HMGB1 and their neutralisation activity was determined in-vitro, with in vivo activity confirmed using a murine model of endotoxemia. Results indicated that CoVaccine HT™ elicited significantly higher antibody tires with stronger affinity and more functional potency than antibodies induced with Freund's adjuvants. These studies provide evidence that CoVaccine HT™ is superior to Freund's adjuvants for the production of antibodies to antigens with low immunogenicity and supports the use of this alternative adjuvant for clinical and experimental use antibodies.