Melissa officinalis efficacy against human influenza virus (New H1N1) in comparison with oseltamivir

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• 作者：Parvane Jalali^a ; Afagh Moattari^b ; Ali Mohammadi^a ; Nima Ghazanfari^c ; Gholamhosein Pourghanbari^d ; ^{hpourghanbari@ardakan.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; ^{hpourghanbari@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Melissa officinalis ; New H1N1 ; Antiviral ; Cell culture
• 刊名：Asian Pacific Journal of Tropical Disease
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：6
• 期：9
• 页码：714-717
• 全文大小：376 K

文摘

To evaluate the antiviral activity of Melissa officinalis (MO) extract against the influenza virus H1N1 in vitro.

Methods

The cytotoxicity of MO extract was identified on Madin-Darby canine kidney (MDCK) cell culture by MTT assay. The virus was inoculated to the cells (multiplicity of infection = 0.1) in two protocols. In protocol 1, the MO extracts at concentrations of 0.005, 0.050, 0.010, 0.100 and 0.500 mg/mL were incubated with the virus for one hour pre-inoculation. In protocol 2, the mentioned concentrations of MO extracts were added to the cells one-hour post infection. Furthermore, the antiviral effect of oseltamivir with different concentrations was tested as the positive controls. The 50% tissue culture infective dose, neutralizing index and hemagglutination titer were determined.

Results

The medicine oseltamivir and MO extracts were not toxic for MDCK at concentrations less than 1 mg/mL. All utilized concentrations of MO extracts were vigorously efficient to decrease the viral yield in both experiments. The 50% tissue culture infective dose of the groups containing up to 0.100 mg/mL of MO extracts in the first experiment in compare with 0.050 mg/mL in the second experiment reduced to 0. Although hemagglutination tests showed little titers, the viral quantity significantly decreased in both experiments. By the way, the medicine oseltamivir could completely suppress viral replication in MDCK.

Conclusions

The present study suggests that MO extracts have a potent anti-influenza effect in cell culture.