In vitro and in vivo anti-inflammatory effects of theaflavin-3,3′-digallate on lipopolysaccharide-induced inflammation

详细信息    查看全文

• 作者：Yanting Wu^a ; ¹ ; Fujun Jin^a ; ¹ ; Yiliang Wang^b ; Feng Li^b ; Lu Wang^a ; Qiaoli Wang^a ; Zhe Ren^a ; Yifei Wang^a ; ^{twang-yf@163.com}
• 关键词：Inflammation ; Theaflavins ; Theaflavin-3 ; 3&prime ; -digallate Lipopolysaccharide ; Acute lung injury
• 刊名：European Journal of Pharmacology
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：794
• 期：Complete
• 页码：52-60
• 全文大小：1234 K
• 卷排序：794

文摘

Inflammation is a defensive response against various harmful stimuli and stress conditions, such as tissue injury and one of the most common pathological processes occurring in human diseases. Theaflavin-3,3′-digallate, one of the theaflavins present in black tea, exhibits several bioactive properties, including the ability to lower the incidence of coronary heart disease, a positive effect on the bone mineral density, and the ability to prevent cancer. The aim of this study was to evaluate whether theaflavin-3,3′-digallate could reduce the production of pro-inflammatory cytokines in vivo and in vitro and ameliorate acute lung injury (ALI) in a mouse model. In this study, we demonstrated that theaflavin-3,3′-digallate suppressed the lipopolysaccharide (LPS)-induced phosphorylation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase in RAW 264.7 macrophages. In addition, we also showed that theaflavin-3,3′-digallate inhibited the expression of tumor necrosis factor alpha, interleukin -1 beta, and interleukin 6 in phorbol myristate acetate -primed U937 and RAW 264.7 cells. Furthermore, theaflavin-3,3′-digallate treatment attenuated the severity of LPS-induced ALI in mice. These results suggested that theaflavin-3,3′-digallate might be a potential therapeutic candidate for the treatment of inflammation and inflammatory diseases.