G protein-coupled receptor mediated sensing of TMA

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• 作者：Anke Suska ; Ana B. Ibá ; ñ ; ez ; Pakorn Preechaburana ; Ingemar Lundstr&#xf6 ; m ; Anna Berghard
• 关键词：Cell-based sensor ; trace amine-associated receptor ; TAAR5 ; trimethylamine detection ; Xenopus laevis melanophores
• 刊名：Procedia Chemistry
• 出版年：2009
• 出版时间：August 2009
• 年：2009
• 卷：1
• 期：1
• 页码：321-324
• 全文大小：621 K

文摘

A new approach for the detection of trimethylamine (TMA) using recombinant Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the olfactory epithelium, which conferred high sensitivity to TMA. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a convenient evaluation of TMA levels in fish tissue extracts. The developed gas measurement platform was able to detect TMA from 1 to 100 ppm within thirty-five minutes.