Effect of the Schiff base complex diaqua-(N-salicylidene-l-glutamato)copper(II) monohydrate on human tumor cells

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• 作者：Katarina Konarikova ; Lucia Andrezalova ; Peter Rapta ; Marianna Slovakova ; Zdenka Durackova ; Lucia Laubertova ; Helena Gbelcova ; Lubomir Danisovic ; Daniel Bohmer ; Tomas Ruml ; Martin Sveda ; Ingrid Zitnanova
• 关键词：Copper(II) complex ; Tumor cell line ; Caspase ; Cytotoxicity
• 刊名：European Journal of Pharmacology
• 出版年：5 December, 2013
• 年：2013
• 卷：721
• 期：1-3
• 页码：178-184
• 全文大小：1133 K

文摘

The aim of our study was to estimate cytostatic/cytotoxic activity of the copper(II) Schiff base complex of the composition [Cu(N-salicylidene-l-glutamato)(H₂O)₂]路H₂O, further Cu(SG-L)H₂O, against human colon carcinoma cell line HT-29, as well as to determine type of cell death and to find out the molecular mechanism of apoptosis induced by this complex. Two highest concentrations (50, 100 碌mol/l) of the complex showed a strong cytotoxic activity against human colon carcinoma cells HT-29 after 72 h of influence. Other concentrations had a cytostatic activity. Unchelated copper(II) ions and free ligands had no effect on the cell growth. Cu(SG-L)H₂O preferentially reduced cancer cell viability compared to healthy cells (NIH-3T3). Cu(SG-L)H₂O induced apoptosis of cells HT-29 at all concentrations used (1-100 碌mol/l) after 48 h of influence. Apoptosis was carried out by the mitochondrial pathway with active caspases 3 and 9. By the spin-trapping technique combined with electron paramagnetic resonance we found that our complex is photochemically stable in aqueous systems and does not exhibit radical-scavenging activity when 2,2鈥?azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation radical was used as an oxidant. The complex exhibits a strong prooxidant property in the initial stages of thermal decomposition of K₂S₂O₈ in water solutions leading to the massive production of ^路OH radicals. Therefore, this complex could strongly participate in anticancer action via a free radical mechanism.