The binding effectiveness of anti-r-disintegrin polyclonal antibodies against disintegrins and PII and PIII metalloproteases: An immunological survey of type A, B and A + B venoms from Mohave rattlesnakes

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• 作者：Esteban Cantú ; Jr.^a ; Sahiti Mallela^a ; Matthew Nyguen^a ; Raú ; l Bá ; ez^a ; Victoria Parra^a ; Rachel Johnson^a ; Kyle Wilson^a ; Montamas Suntravat^a ; Sara Lucena^a ; Alexis Rodrí ; guez-Acosta^b ; Elda E. Sá ; nchez^a ; ^c ; ^{elda.sanchez@tamuk.edu}
• 关键词：SVMP ; snake venom metalloprotease ; ELISA ; Enzyme-Linked Immunosorbent Assay ; ARDPA ; anti-recombinant disintegrin polyclonal antibody ; r-Mojastin 1 ; recombinant mojastin 1 ; ADAM ; a disintegrin and metalloprotease ; LD50 ; Lethal Dose 50 ; LC-MS ; Liquid Chromatography-Mass Spectrometry ; IACUC ; Institutional Animal Care and Use Committee ; ACT ; Activated Clot Time ; CR ; Clot Rate ; PF ; Platelet Function ; gbACT  ; +  ; Kit ; glass bead activated cuvette kit
• 刊名：Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：191
• 期：Complete
• 页码：168-176
• 全文大小：1389 K
• 卷排序：191

文摘

Snake venoms are known to have different venom compositions and toxicity, but differences can also be found within populations of the same species contributing to the complexity of treatment of envenomated victims. One of the first well-documented intraspecies venom variations comes from the Mohave rattlesnake (Crotalus scutulatus scutulatus). Initially, three types of venoms were described; type A venom is the most toxic as a result of ~ 45% Mojave toxin in the venom composition, type B lacks the Mojave toxin but contains over 50% of snake venom metalloproteases (SVMPs). Also, type A + B venom contains a combination of Mojave toxin and SVMP. The use of an anti-disintegrin antibody in a simple Enzyme-Linked Immunosorbent Assay (ELISA) can be used to identify the difference between the venoms of the type A, B, and A + B Mohave rattlesnakes. This study implements the use of an anti-recombinant disintegrin polyclonal antibody (ARDPA) for the detection of disintegrins and ADAMs (a disintegrin and metalloproteases) in individual crude snake venoms of Mohave rattlesnakes (Crotalus scutulatus scutulatus) of varying geographical locations. After correlation with Western blots, coagulation activity and LD50 data, it was determined that the antibody allows for a quick and cost-efficient identification of venom types.