Appropriate real-time PCR reference genes for fluoride treatment studies performed in vitro or in vivo
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- 作者：D. Faibish dfaibish@forsyth.org" class="auth_mail" title="E-mail the corresponding author ; M. Suzuki1 ; suzuki.94@osu.edu" class="auth_mail" title="E-mail the corresponding author ; J.D. Bartlett ; bartlett.196@osu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：AAALAC ; Association for Assessment and Accreditation of Laboratory Animal Care International ; Cq ; quantification cycle ; IACUC ; Institutional Animal Care Use Committee ; qPCR ; quantitative real-time PCR
- 刊名：Archives of Oral Biology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：62
- 期：Complete
- 页码：33-42
- 全文大小：1316 K
文摘
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Identification of reference genes for fluoride qPCR experiments is proposed.
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RNA was isolated from LS8 ameloblast-derived cells and rat enamel organs.
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qPCR experiments must first validate the stability of reference gene expression in context of the specified conditions.
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Gapdh and Eef1a1 were the most stably expressed genes in enamel organs from fluoride-treated rats.