Functional role of mitochondrial respiratory supercomplexes

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• 作者：Maria Luisa Genova ; Giorgio Lenaz
• 关键词：AOX ; alternative oxidase ; BN-PAGE ; Blue-Native gel electrophoresis ; CI ; Complex I ; CII ; Complex II ; CIII ; Complex III ; CIV ; Complex IV ; CJ ; cristae junction ; CoQ ; Coenzyme Q ; Ubiquinone ; CREB ; response element binding protein ; ETF ; electron transfer flavoprotein ; mtDNA ; mitochondrial DNA ; OXPHOS ; oxidative phosphorylation ; ROS ; reactive oxygen species
• 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
• 出版年：April, 2014
• 年：2014
• 卷：1837
• 期：4
• 页码：427-443
• 全文大小：1101 K

文摘

Recent experimental evidence has replaced the random diffusion model of electron transfer with a model of supramolecular organisation based upon specific interactions between individual respiratory complexes. These supercomplexes were found to be functionally relevant by flux control analysis and to confer a kinetic advantage to NAD-linked respiration (channelling). However, the Coenzyme Q pool is still required for FAD-linked oxidations and for the proper equilibrium with Coenzyme Q bound in the supercomplex. Channelling in the cytochrome c region probably also occurs but does not seem to confer a particular kinetic advantage. The supramolecular association of individual complexes strongly depends on membrane lipid amount and composition and is affected by lipid peroxidation; it also seems to be modulated by membrane potential and protein phosphorylation. Additional properties of supercomplexes are stabilisation of Complex I, as evidenced by the destabilising effect on Complex I of mutations in either Complex III or IV, and prevention of excessive generation of reactive oxygen species. The dynamic character of the supercomplexes allows their involvement in metabolic adaptations and in control of cellular signalling pathways. This article is part of a Special Issue entitled: Dynamic and ultrastructure of bioenergetic membranes and their components.