Structure and Orientation of a Voltage-Sensor Toxin in Lipid Membranes

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• 作者：Hyun Ho Jung ; Hoi Jong Jung ; Mirela Milescu ; Chul Won Lee ; Seungkyu Lee ; Ju Yeon Lee ; Young-Jae Eu ; Ha Hyung Kim ; Kenton J. Swartz ; Jae Il Kim
• 刊名：Biophysical Journal
• 出版年：2010
• 出版时间：21 July 2010
• 年：2010
• 卷：99
• 期：2
• 页码：638-646
• 全文大小：1105 K

文摘

Amphipathic protein toxins from tarantula venom inhibit voltage-activated potassium (Kv) channels by binding to a critical helix-turn-helix motif termed the voltage sensor paddle. Although these toxins partition into membranes to bind the paddle motif, their structure and orientation within the membrane are unknown. We investigated the interaction of a tarantula toxin named SGTx with membranes using both fluorescence and NMR spectroscopy. Depth-dependent fluorescence-quenching experiments with brominated lipids suggest that Trp³⁰ in SGTx is positioned 9 Å from the center of the bilayer. NMR spectra reveal that the inhibitor cystine knot structure of the toxin does not radically change upon membrane partitioning. Transferred cross-saturation NMR experiments indicate that the toxin's hydrophobic protrusion contacts the hydrophobic core of the membrane, whereas most surrounding polar residues remain at interfacial regions of the bilayer. The inferred orientation of the toxin reveals a twofold symmetry in the arrangement of basic and hydrophobic residues, a feature that is conserved among tarantula toxins. These results have important implications for regions of the toxin involved in recognizing membranes and voltage-sensor paddles, and for the mechanisms by which tarantula toxins alter the activity of different types of ion channels.