PI3K pathway is involved in ERK signaling cascade activation by histamine H2R agonist in HEK293T cells
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- 作者：Natalia Alonsoa ; 1 ; Antonela Diaz Nebredaa ; Federico Monczorb ; c ; J. Silvio Gutkindd ; 2 ; Carlos Daviob ; c ; Natalia Fernandezb ; c ; Carina Shayoa ; carinashayo@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; cshayo@dna.uba.ar" class="auth_mail" title="E-mail the corresponding author
- 关键词：HR ; histamine receptor ; GPCR ; G-protein coupled-receptor ; ERK1/2 ; extracellular signal-regulated kinases ; PI3K ; phosphoinositide 3-kinase ; DMEM ; Dulbecco's modified Eagle's medium ; PBS ; phosphate-buffered saline ; DMEM/F12 ; Dulbecco's modified Eagle's medium/Ham F12 ; ECL ; enhanced chemiluminescence ; IBMX ; 1-methyl-3-isobutylxanthine ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; DNDyn ; dynamin dominant negative mutant ; Akt-myr ; constitutive active mutant of Akt1 ; PKI ; PKA p
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1860
- 期：9
- 页码：1998-2007
- 全文大小：1263 K
文摘
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PI3K/Akt and ERK cascade are crossregulated by H2R activation.
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10">H2R stimulation inhibits PI3K/Akt/mTOR and activates Ras/MEK/ERK pathways in H2R-transfected HEK293T cells.
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15">PI3K/Akt/mTOR signaling inhibition is necessary to achieve H2R mediated ERK activation.
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Amthamine-induced proliferation mediated by ERK activation in Leydig MA-10 cells was enhanced by LY294002, a PI3K inhibitor.