Construction and co-expression of polycistronic plasmids encoding thermophilic l-arabinose isomerase and hyperthermophilic β-galactosidase for single-step production of d-tagatose
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- 作者：Zheng Xua ; b ; xuzheng@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhaoxian Xua ; b ; Bao Tanga ; b ; Sha Lia ; b ; Jian Gaoc ; Bo Chia ; b ; Hong Xua ; b ; xuh@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：l-Arabinose isomerase ; d-Tagatose ; Biocatalysis ; Enzymes ; Lactose ; Bioprocess design
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：109
- 期：Complete
- 页码：28-34
- 全文大小：988 K
文摘
AI from L. fermentum and β-galactosidase from T. thermophilus were co-expressed.
101 g/L lass="smallcaps">l-ribose with yield of 20.2% and productivity of 6.3 g/L h was obtained.
The orders of the encoding genes, SD, and AS length were optimized.
Cells showed balanced activity for two enzymes using optimized SD and AS sequence.