Top-down protein identification using isotopic envelope fingerprinting

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• 作者：Kaijie Xiao^a ; ^b ; Fan Yu^a ; ^b ; Zhixin Tian^a ; ^b ; ^{zhixintian@tongji.edu.cn}
• 关键词：Protein identification ; Intact protein ; Intact proteome ; Top-down ; Isotopic envelope fingerprinting ; Protein database search ; Post-translational modifications ; Protein species ; Proteoform ; UVPD
• 刊名：Journal of Proteomics
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：152
• 期：Complete
• 页码：41-47
• 全文大小：848 K
• 卷排序：152

文摘

Interpreting protein mass spectra directly using isotopic envelopes instead of “deisotoped” masses FDR control with decoy database search Comprehensive handling of amino acid variation, dynamic PTMs and static chemical labeling Comprehensive leverage of collision-, electron-, and photon-based dissociation as well as various types of product ions in each dissociation method User friendly GUIs, search results output & visualization