Enhancing analysis throughput, sensitivity and specificity in LC/ESI-MS/MS assay of plasma 25-hydroxyvitamin D₃ by derivatization with triplex 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) isotopologues

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• 作者：Shoujiro Ogawa^a ; Hiroki Kittaka^a ; Akiho Nakata^a ; Kenji Komatsu^b ; Takahiro Sugiura^b ; Mamoru Satoh^c ; Fumio Nomura^c ; Tatsuya Higashi^a ; ^{higashi@rs.tus.ac.jp}
• 关键词：LC/ESI&ndash ; MS/MS ; Derivatization ; Isotopologue ; Analysis throughput ; Vitamin D
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 March 2017
• 年：2017
• 卷：136
• 期：Complete
• 页码：126-133
• 全文大小：605 K
• 卷排序：136

文摘

An enhanced throughput LC/ESI–MS/MS method was developed for the 25(OH)D3 measurement. The method employed the derivatization with the triplex DAPTAD isotopologues. Use of isotopologues enabled the analyses of 3 different samples within a single run. The method was successfully applied to the analysis of adult and infant plasma samples.