Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons

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• 作者：Vsevolod Bodrikov^a ; ^{Vsevolod.Bodrikov@uni-konstanz.de} ; Aline Pauschert^a ; ^{AlinePauschert@web.de} ; Gaga Kochlamazashvili^b ; ^{kochlamazashvili@fmp-berlin.de} ; Claudia A.O. Stuermer^a ; ^{Claudia.Stuermer@uni-konstanz.de}
• 关键词：CA ; constitutive active ; ECS ; extracellular solution ; EGFP ; enhanced green fluorescent protein ; EHD ; Eps15 homology domain-containing proteins ; Flot2 ; flotillin-2 ; k.o. ; knock out ; LTP ; long term potentiation ; MAPK ; mitogen-activated kinase ; div ; days in culture ; GluN1 ; NMDA receptor subunit 1 (NMDA receptor ; N-Methyl-d-Aspartate receptor) ; GluA1 ; glutamate receptor subunit 1 (AMPA receptor ; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) ; RFP ; red fluorescent protein ; FM4-64FX ; f
• 刊名：Experimental Neurology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：289
• 期：Complete
• 页码：31-45
• 全文大小：2409 K
• 卷排序：289

文摘

Reggies (flotillins) and Rab11a regulate trafficking of postsynaptic cargo. Reggies deliver PSD-95, N-cadherin, GluA1 and GluN1 to the postsynaptic spine. Reggie-deficient hippocampal neurons suffer from impaired cargo trafficking. Reggie downregulation impairs spine synapse formation. Surface exposure of GluA1 and GluN1 is reduced which impairs in vitro LTP.