Prostaglandin F_2α stimulates tyrosine phosphorylation of phospholipase C-γ1

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• 作者：Husain ; Shahid ; Jafri ; Farahdiba
• 关键词：Phospholipase C-γ ; 1 ; Tyrosine phosphorylation ; Prostaglandin F_2α ; ; Non-vascular smooth muscle
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2002
• 出版时间：October 11, 2002
• 年：2002
• 卷：297
• 期：5
• 页码：1102-1107
• 全文大小：191 K

文摘

In this study, we investigated the ability of prostaglandin F_2α (PGF_2α) to induce tyrosine phosphorylation of phospholipase C-γ1 (PLC-γ1) in cat iris sphincter smooth muscle (CISM) cells. PGF_2α(1μM) stimulated PLC-γ1 tyrosine phosphorylation in a time- and dose-dependent manner with a maximum increase of 3-fold at 0.5min. The protein tyrosine kinase inhibitors, genistein, and tyrphostin A-25, blocked the stimulatory effects of PGF_2α, suggesting involvement of protein tyrosine kinase activity in the physiological actions of the PGF_2α. Furthermore, PGF_2α-induced p42/p44 MAP kinase activation was also completely blocked by protein tyrosine kinase inhibitors. In summary, these findings show that PGF_2α stimulates tyrosine phosphorylation of PLC-γ1 in CISM cells and indicate that PGF_2α-stimulated tyrosine phosphorylation is responsible for an early signal transduction event.