• 作者：Eli¨² Elgorriaga-Islas ; Piero Guggiana-Nilo ; Mariana Dom¨ªnguez-Y¨¦venes ; Gerardo Gonz¨¢lez-Rocha ; Sergio Mella-Montecinos ; Jaime Labarca-Labarca ; Patricia Garc¨ªa-Ca?ete ; Helia Bello-Toledo
• 关键词：aac(6&rsquo ; )-Ib ; aac(6&rsquo ; )-Ib-cr ; Klebsiella pneumoniae ; Escherichia coli ; Determinantes plasmí ; dicos de resistencia a quinolonas (DPRQ) ; Quinolona ; Chile
• 刊名：Enfermedades Infecciosas y Microbiolog¨ªa Cl¨ªnica
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：30
• 期：8
• 页码：466-468
• 全文大小：202 K

lass=""h4"">Introduction

The frequency of aac(6¡ä)-Ib-cr gene in ESBL-producing strains of Klebsiella pneumoniae and Escherichia coli is unknown, in Chile.

lass=""h4"">Methodology

The aac(6¡ä)-Ib and aac(6¡¯)-Ib-cr genes were investigated using polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and sequencing, in strains isolated from 10 Chilean hospitals between 2008-2009.

lass=""h4"">Results

The aac(6¡¯)-Ib-cr gene was detected in 54 % of K. pneumoniae and 74 % of E. coli strains. The CIM₅₀ of CIP was higher among strains harboring aac(6¡¯)-Ib-cr, 8 times higher in K. pneumoniae and 4 times higher in E. coli. Moreover, both aac(6¡¯)-Ib and aac(6¡¯)-Ib-cr were simultaneously found in 13 K. pneumoniae and 3 E. coli isolates.

lass=""h4"">Conclusion

This is the first report of aac(6¡¯)-Ib-cr in ESBL-producing strains of K. pneumoniae and E. coli isolated from in-patients in Chilean hospitals located along an area of more than 2,800 Km.