Taurine treatment preserves brain and liver mitochondrial function in a rat model of fulminant hepatic failure and hyperammonemia

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• 作者：Akram Jamshidzadeh^a ; ^b ; Reza Heidari^a ; ^{rezaheidari@hotmail.com} ; Mozhgan Abasvali^c ; Mehdi Zarei^b ; Mohammad Mehdi Ommati^d ; Narges Abdoli^e ; Forouzan Khodaei^b ; Yasaman Yeganeh^b ; Faezeh Jafari^b ; Azita Zarei^b ; Zahra Latifpour^b ; Elnaz Mardani^b ; Negar Azarpira^f ; Behnam Asadi^b ; Asma Najibi^b
• 关键词：Amino acid ; Ammonia ; Bioenergetics ; Brain injury ; Hepatic encephalopathy ; Oxidative stress
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：86
• 期：Complete
• 页码：514-520
• 全文大小：891 K
• 卷排序：86

文摘

Ammonia-induced mitochondrial dysfunction and energy crisis is known as a critical consequence of hepatic encephalopathy (HE). Hence, mitochondria are potential targets of therapy in HE. The current investigation was designed to evaluate the role of taurine treatment on the brain and liver mitochondrial function in a rat model of hepatic encephalopathy and hyperammonemia. The animals received thioacetamide (400 mg/kg, i.p, for three consecutive days at 24-h intervals) as a model of acute liver failure and hyperammonemia. Several biochemical parameters were investigated in the serum, while the animals’ cognitive function and locomotor activity were monitored. Mitochondria was isolated from the rats’ brain and liver and several indices were assessed in isolated mitochondria. Liver failure led to cognitive dysfunction and impairment in locomotor activity in the rats. Plasma and brain ammonia was high and serum markers of liver injury were drastically elevated in the thioacetamide-treated group. An assessment of brain and liver mitochondrial function in the thioacetamide-treated animals revealed an inhibition of succinate dehydrogenase activity (SDA), collapsed mitochondrial membrane potential, mitochondrial swelling, and increased reactive oxygen species (ROS). Furthermore, a significant decrease in mitochondrial ATP was detected in the brain and liver mitochondria isolated from thioacetamide-treated animals. Taurine treatment (250, 500, and 1000 mg/kg) decreased mitochondrial swelling, ROS, and LPO. Moreover, the administration of this amino acid restored brain and liver mitochondrial ATP. These data suggest taurine to be a potential protective agent with therapeutic capability against hepatic encephalopathy and hyperammonemia-induced mitochondrial dysfunction and energy crisis.