Molecular diversity of human parvovirus B19 during two outbreaks of erythema infectiosum in Brazil

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• 作者：Rita de Cá ; ssia Nasser Cubel Garcia^a ; ^{ritacubel@id.uff.br} ; Renata Freire Alves Pereira^a ; ^b ; Ká ; tia Martins Lopes de Azevedo^c ; Tatiana Xavier de Castro^a ; Francisco C.A. Mello^a ; Sé ; rgio Setubal^c ; Marilda M. Siqueira^d ; David Brown^d ; Solange Artimos de Oliveira^b ; ^c
• 关键词：B19V genotypes ; Outbreak ; Erythema infectiosum ; Brazil
• 刊名：The Brazilian Journal of Infectious Diseases
• 出版年：2017
• 出版时间：January-February 2017
• 年：2017
• 卷：21
• 期：1
• 页码：102-106
• 全文大小：966 K
• 卷排序：21

文摘

This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996–2006, a period with two distinct outbreaks of B19V infection: 1999–2000 and 2004–2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). The phylogenetic tree supported the division of the G1a into two well-defined clades with 1.3% of divergence. The low diversity of the G1a strains may be explained by the fact that all patients had acute B19V infection and 30/32 sera were collected during two distinct outbreaks. The G3b strain was from an HIV-infected patient who seroconverted to anti-B19 IgG antibodies in September/2005. This is the first report of G3b in the state of Rio de Janeiro.