Spectroscopic identification of AZT derivative obtained from biotransformation of AZT by Stenotrophomonas maltophilia
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- 作者：Kruszewska ; Hanna ; Chmielowiec ; Urszula ; Bednarek ; Elzbieta ; Witowska-Jarosz ; Janina ; Dobrowolski ; Jan Cz. ; et. al.
- 关键词：3&prime ; -Azido-2&prime ; 3&prime ; -dideoxy-β ; -ribosylthymine ; 3&prime ; -Azido-3&prime ; -deoxy-β ; -ribosylthymine ; AZT ; Stenotrophomonas maltophilia ; MS ; NMR ; IR ; Raman
- 刊名：Journal of Molecular Structure
- 出版年：2003
- 出版时间：June 1, 2003
- 年：2003
- 卷：651-653
- 期：Complete
- 页码：645-650
- 全文大小：105 K
文摘
The 3′-azido-2′,3′-dideoxy-β-ribosylthymine (AZT, Zidovudine) is a cytostatic antivirial drug worldwide used in AIDS treatment or, in combination with other antiproliferative drugs, in treatment of cancer. About 30–40 % of AZT is metabolised by conjunction with glucuronic acid in liver and about 70 % is eliminated untouched by urinary system. In this work a possible fate of the AZT in the environment is studied. To this end, a product of AZT biotransformation by an environmental strain, Stenotrophomonas maltophilia, (aerobic, Gram(−) rod, common in soil and water) is found and isolated by HPLC and TLC techniques and identified by NMR and mass spectroscopy. All the molecular spectroscopy methods confirm presence of the product, which is AZT molecule hydroxylated in the position 2′ of the deoxyribose ring.