HPLC analysis and standardization of Brahmi vati - An Ayurvedic poly-herbal formulation

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• 作者：Amrita Mishra ; Arun K. Mishra ; Om Prakash Tiwari ; Shivesh Jha
• 关键词：Brahmi vati ; Bacoside A3 ; Piperine ; HPLC
• 刊名：Journal of Young Pharmacists
• 出版年：September, 2013
• 年：2013
• 卷：5
• 期：3
• 页码：77-82
• 全文大小：618 K

文摘

ss="h4">Objectives

The aim of the present study was to standardize Brahmi vati (BV) by simultaneous quantitative estimation of Bacoside A<sub>3sub> and Piperine adopting HPLC-UV method. BV very important Ayurvedic polyherbo formulation used to treat epilepsy and mental disorders containing thirty eight ingredients including Bacopa monnieri L. and Piper longum L.

ss="h4">Materials and methods

An HPLC-UV method was developed for the standardization of BV in light of simultaneous quantitative estimation of Bacoside A<sub>3sub> and Piperine, the major constituents of B.聽monnieri L. and P.聽longum L. respectively. The developed method was validated on parameters including linearity, precision, accuracy and robustness.

ss="h4">Results

The HPLC analysis showed significant increase in amount of Bacoside A<sub>3sub> and Piperine in the in-house sample of BV when compared with all three different marketed samples of the same. Results showed variations in the amount of Bacoside A<sub>3sub> and Piperine in different samples which indicate non-uniformity in their quality which will lead to difference in their therapeutic effects.

ss="h4">Conclusion

The outcome of the present investigation underlines the importance of standardization of Ayurvedic formulations. The developed method may be further used to standardize other samples of BV or other formulations containing Bacoside A<sub>3sub> and Piperine.