The particle size and PDI value of Rehmannia glutinosa polysaccharide liposome encapsulated with PCV-2 showed little change within 30 days, which indicated its good stability.
In vivo, Rehmannia glutinosa polysaccharide liposome could elicit a strong IgG response and significantly increased the production of Th1 and Th2 associated IgG subtypes and cytokines.
Rehmannia glutinosa polysaccharide liposome-activated of macrophages exhibited enhanced phagocytic activity, and secreted significant higher concentration of IL-1β, TNF-α, IFN-γ, IL-6, and IL-12 than RGP did.
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