Optimization on preparation conditions of Rehmannia glutinosa polysaccharide liposome and its immunological activity

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• 作者：Yee Huang^a ; ¹ ; Caihong Wu^b ; ¹ ; Zhenguang Liu^a ; Yuanliang Hu^a ; Cheng Shi^a ; Yun Yu^a ; Xiaojuan Zhao^a ; Cui Liu^a ; Jiaguo Liu^a ; Yi Wu^a ; Deyun Wang^a ; ^{dywang@njau.edu.cn" class="auth_mail}
• 关键词：RGPL ; Rehmannia glutinosa polysaccharide liposome ; RGP ; Rehmannia glutinosa polysaccharide ; RSM ; response surface methodology ; PHA ; phytohemagglutinin ; LPS ; lipopolysaccharide ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; PBS ; phosphate buffered saline ; DMSO ; dimethyl sulfoxide ; EE ; entrapment efficiency ; BBD ; Box&ndash ; Behnken design ; BL ; blank liposome
• 刊名：Carbohydrate Polymers
• 出版年：15 April, 2014
• 年：2014
• 卷：104
• 期：Complete
• 页码：118-126
• 全文大小：2446 K

文摘

The aim of this study was to investigate the optimizing preparation conditions and immunological enhancement activity of Rehmannia glutinosa polysaccharide liposome (RGPL). RGPL was prepared using the reverse-phase evaporation method and optimized using the response surface methodology. The immunological enhancement activity of RGPL on splenocyte proliferation was measured. These results showed that the optimum preparation conditions were: a soybean phosphatide to cholesterol ratio of 8:1, a chloroform to methanol ratio of 3:5, a soybean phosphatide to tween 80 ratio of 10:1, a temperature (掳C) of 66 掳C and an entrapment efficiency of 72.753 卤 0.318%. In a single stimulation of drugs, RGPL could significantly promote splenocyte proliferation, specifically at 200 渭g mL^鈭?. Moreover, in a synergistic stimulation of drugs with LPS or PHA, a significant difference was obtained between the RGPL and RGP at 100-400 渭g mL^鈭?, which indicated that the immunological enhancement of RGP was significantly improved after encapsulation with the liposome.