RNA interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus
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- 作者：Xiao Wang1 ; Yongfeng Li1 ; Lian-Feng Li1 ; Liang Shen ; Lingkai Zhang ; Jiahui Yu ; Yuzi Luo ; Yuan Sun ; Su Li ; Hua-Ji Qiu ; huajiqiu@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; qiuhuaji@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Classical swine fever virus ; Interferon stimulated genes ; Reporter virus ; Small interfering RNAs ; Antiviral activities
- 刊名：Antiviral Research
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：49-56
- 全文大小：1094 K
文摘
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A reporter classical swine fever virus (CSFV) stably expressing the Renilla luciferase was generated.
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The reporter virus showed a growth curve indistinguishable from that of the parent virus.
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The reporter virus together with siRNAs was used to screen anti-CSFV ISGs from 21 candidates.
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Four potent anti-CSFV ISGs, NRAMP1, NT5C3A, OAS1, and CXCL10, were identified and verified.