Identification of two novel female-specific DNA sequences in half-smooth tongue sole, Cynoglossus semilaevis

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• 作者：Xubo Wang¹ ; Jiajun Jiang¹ ; Jinning Gao ; Jinxiang Liu ; Jie Qi ; Zhigang Wang ; Haiyang Yu ; Quanqi Zhang ; ^{qzhang@ouc.edu.cn}
• 关键词：Female-specific sequence ; Cynoglossus semilaevis ; Half-smooth tongue sole ; Fluorescent in situ hybridization ; Sex identification
• 刊名：Aquaculture
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：388-391
• 期：Complete
• 页码：49-53
• 全文大小：903 K

文摘

Half-smooth tongue sole (Cynoglossus semilaevis) is a kind of cultured teleost fish with larger size of females than males. In order to produce all-female progeny, it is necessary to distinguish the genetic females and males precisely. In this study, a W chromosome library, which had been constructed by laser microdissection of W chromosomes, was screened. Among four hundreds and twelve pairs of designed PCR primers, two pairs (W045 and W131) could produce female-specific fragments in fifty individuals. To verify the primers and optimize the method, another one hundred individuals were tested by multiplex PCR with both W045 and a pair of positive control primers. Furthermore, two sex-reversed individuals were also identified correctly. Fluorescent in situ hybridization (FISH) further confirmed that fragments form W045 and W131 primers only localized to the W chromosome in female individuals, and the FISH technique with the two female-specific fragments could help to isolate super-female individuals. The identification of two novel female-specific DNA sequences and the techniques used here may be applied to distinguish the genetic gender of half-smooth tongue sole precisely. Besides, these also help us to obtain a better understanding of fish sex determination.