Two novel pH probes (boldFont">L1 and boldFont">L2) were facilely synthesized by one-step reaction.
Fluorescence intensity ratio of boldFont">L1 and boldFont">L2 can quantify the pH fluctuations.
boldFont">L1 and boldFont">L2 show high quantum yield and large two-photon absorption cross-section.
boldFont">L1 and boldFont">L2 show excellent selectivity response and remarkable color change to H+.
boldFont">L1 and boldFont">L2 were successfully utilized to mapping pH difference intracellularly.