NH3-N, TN, TP, and COD in actual wastewater were removed by DNPAOs efficiently.
Separation of sludge and wastewater improved metabolic activity of DNPAOs.
Isolated bacterium is identified as a non-fermentative strain, Pseudomonas aeruginosa.
Intracellular PHB served as energy source and carbon source in Pseudomonas aeruginosa.