Novel spectrophotometric approach for determination of validamycin A in fermentation of Streptomyces hygroscopicus

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• 作者：Wei Li ^{jiangnanliwei2008@163.com} ; Jinsong Feng ^{fjs061@163.com} ; Yan Liu ^{wsliuyan@163.com} ; Jing Jiang ^{2234294785@qq.com} ; Xiaodong Zheng ^{xdzheng@zju.edu.cn} ; Wen-Wen Zhou ; ^{vivianzhou11@zju.edu.cn}
• 关键词：High performance liquid chromatography analysis ; Pigment ; Spectrophotometry ; Streptomyces ; Validamycin A
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：122
• 期：6
• 页码：736-739
• 全文大小：647 K
• 卷排序：122

文摘

A stable relationship between pigment secretion and Val-A production was found. An equation with SA value of pigment to determine Val-A was fitted. Accuracy and applicability of Spectrophotometry was proved in determining Val-A productivity and screening high-yield mutants. Spectrophotometry assay is reported first as a rapid method in antibiotic fermentation.