Enantioseparation of racemic trans-未-viniferin using high speed counter-current chromatography based on induced circular dichroism technology

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• 作者：Chao Han ; Jinfang Xu ; Xiaobing Wang ; Xiaoming Xu ; Jianguang Luo ; Lingyi Kong
• 关键词：Chiral separation ; High speed counter-current chromatography ; Trans-未-viniferin ; Induced circular dichroism spectrum ; Hydroxypropyl-尾-cyclodextrin
• 刊名：Journal of Chromatography A
• 出版年：10 January, 2014
• 年：2014
• 卷：1324
• 期：Complete
• 页码：164-170
• 全文大小：1195 K

文摘

A preparative chiral high speed counter-current chromatography (HSCCC) method based on induced circular dichroism (ICD) spectrum was developed to separate trans-未-viniferin (TVN) enantiomers successfully. The important parameters for the chiral HSCCC separation process, including the type of chiral selector (CS), the concentration of chiral selector and the equilibrium temperature, were optimized using induced circular dichroism spectrum. The final separation procedure was established with a biphasic solvent system composed of n-hexane-ethyl acetate-25 mmol L^鈭? hydroxypropyl-尾-cyclodextrin aqueous solution (5:5:10, v/v/v) in the head-to-tail elution mode at 5 掳C. Under optimum chiral HSCCC separation conditions, 8.2 mg of (7S, 8S)-TVN (1) and 9.4 mg of (7R, 8R)-TVN (2) were successfully separated from 20 mg TVN enantiomers with the purity of 99.51% and 99.36%, respectively.