3,3′-Diindolylmethane induces anti-human gastric cancer cells by the miR-30e-ATG5 modulating autophagy

详细信息    查看全文

• 作者：Yang Ye^a ; ¹ ; Yanfei Fang^a ; ^b ; ¹ ; Wenxia Xu^c ; Qiang Wang^d ; Jianwei Zhou^d ; Rongzhu Lu^a ; ^e ; ^{lurz@mail.ujs.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：DIM ; 3 ; 3&prime ; -diindolylmethane ; LC3 ; microtubule-associated protein light chain ; ATG ; autophagy-related gene ; mRFP ; monomeric red fluorescent protein ; GFP ; green fluorescent protein ; siRNA ; small interfering RNA ; mRNA ; messenger RNA ; miRNA ; microRNA ; MREs ; miRNA-response elements ; 3-MA ; 3-methyladenine ; BafA1 ; bafilomycin A1 ; DMSO ; dimethyl sulfoxide ; PBS ; phosphate buffer saline ; RT-PCR ; reverse transcription PCR ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; PI3K ; phosphatidylinositol 3-k
• 刊名：Biochemical Pharmacology
• 出版年：2016
• 出版时间：1 September 2016
• 年：2016
• 卷：115
• 期：Complete
• 页码：77-84
• 全文大小：2236 K

文摘

3,3′-Diindolylmethane (DIM), a class of relatively non-toxic indole derivatives from cruciferous vegetables, has been reported as a promising anticancer phytochemical, but the underlying molecular mechanism is not completely elucidated. In the present study we report a novel regulation of autophagy by DIM in human gastric cancer cells. We found that DIM dose-dependently inhibited the growth of gastric cancer cells in vitro and in vivo. Moreover, ATG5 and LC3 were activated by DIM in gastric cancer cells. Furthermore, miR-30e was down-regulated by DIM and miR-30e targeted the 3′-UTR of ATG5 to inhibit its translation. Overall, these results suggest that DIM may through the miR-30e-ATG5 modulating autophagy inhibit the proliferation of gastric cancer cells.