- 作者：Wenhua Zhang ; Jibin Xing ; Dezhao Liu ; Xiaoliang Gan ; Wanling Gao ; Ziqing Hei
- 关键词：Dexamethasone ; Intestine ; Ischemia&ndash ; reperfusion injury ; Mast cell
- 刊名：Journal of Surgical Research
- 出版年：December, 2013
- 年：2013
- 卷：185
- 期：2
- 页码：851-860
- 全文大小：2479 K
Background
Activated mast cells are involved in the pathogenesis of intestinal ischemia-reperfusion (I/R)-related injury. Dexamethasone has been widely used to protect organs from I/R injury. This study was conducted to investigate the impact of treatment with dexamethasone at different stages of the II/R process on mast cell infiltration and activity and intestinal injury.Methods
Kunming mice were randomized and subjected to a sham surgery or the II/R induction by clamping the superior mesenteric artery for 30聽min and then reperfusion. During the II/R induction, the mice were treated intravenously with dexamethasone (10聽mg/kg) for 30聽min before ischemia (pretreatment group), at 5聽min after clamping the superior mesenteric artery (isc-treatment group), or at the beginning of perfusion (rep-treatment group), respectively. The levels of intestinal injury, mast cell infiltration and activity, tumor necrosis factor 伪 (TNF伪) and myeloperoxidase (MPO) activity in the intestines, and mouse survival rates were measured.
Results
The death rates, levels of intestinal injury, mast cell infiltration and activity, and tumor necrosis factor 伪 and myeloperoxidase activity in the intestinal tissues from the II/R group were similar to those from the isc-treatment and rep-treatment groups of mice and were significantly higher than those from the sham group. In contrast, pretreatment with dexamethasone significantly mitigated the II/R-induced mast cell infiltration and activity, inflammation, and intestinal injury and reduced the death rates in mice.
Conclusions
Pretreatment with dexamethasone inhibits II/R injury by reducing mast cell-related inflammation in mice.