Cell-penetrating peptides mediated protein cross-membrane delivery and its use in bacterial vector vaccine
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- 作者：Jimei Ma ; Jinmei Xu ; Lingyu Guan ; Tianjian Hu ; Qin Liu ; Jingfan Xiao ; jfxiao@ecust.edu.cn" class="auth_mail ; cbema307@163.com" class="auth_mail ; Yuanxing Zhang
- 关键词：Cell-penetrating peptides ; Antigen delivery ; Edwardsiella tarda ; Bacterial vector vaccine
- 刊名：Fish & Shellfish Immunology
- 出版年：July 2014
- 年：2014
- 卷：39
- 期：1
- 页码：8-16
- 全文大小：1597 K
文摘
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Cell-penetrating peptides were applied to develop bacterial vector vaccine.
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Several CPPs (TAT, etc.) showed efficient capacity to translocate EGFP into J774 A.1.
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A novel bacterial vector vaccine BL21/pUTa-E + pET28a-FT was established.
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The vaccine candidate presented ideal immune protection in zebrafish (RPS = 63%).
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The humoral and cellular immune responses were both activated by this vector vaccine.