Human turbinate mesenchymal stromal cell sheets with bellows graft for rapid tracheal epithelial regeneration

详细信息    查看全文

• 作者：Jeong Hun Park^a ; ¹ ; Ju Young Park^b ; ¹ ; Inn-Chul Nam^c ; Se-Hwan Hwang^c ; Choung-Soo Kim^c ; Jin Woo Jung^a ; Jinah Jang^b ; Hyungseok Lee^a ; Yeongjin Choi^b ; Sun Hwa Park^c ; Sung Won Kim^c ; ^d ; ^{kswent@catholic.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Dong-Woo Cho^a ; ^{dwcho@postech.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tracheal epithelial regeneration ; Human turbinate mesenchymal stromal cell (hTMSC) sheets ; Indirect three-dimensional (3D) printing ; Tracheal bellows graft ; Tissue engineering
• 刊名：Acta Biomaterialia
• 出版年：2015
• 出版时间：1 October 2015
• 年：2015
• 卷：25
• 期：Complete
• 页码：56-64
• 全文大小：3192 K

文摘

Rapid functional epithelial regeneration on the luminal surface is essential when using artificial tracheal grafts to repair tracheal defects. In this study, we imposed human turbinate mesenchymal stromal cell (hTMSC) sheets for tracheal epithelial regeneration, and then assessed their potential as a new clinical cell source. In vitro, hTMSCs sheets showed high capacity to differentiate into tracheal epithelium. We fabricated a poly(蔚-caprolactone) (PCL) tracheal graft by indirect three-dimensional (3D) printing technique and created a composite construct by transplanting the hTMSC sheets to its luminal surface of the tracheal graft, then applied this tissue-engineered tracheal graft to non-circumferential tracheal reconstruction in a rabbit model. 4 weeks after implantation, the luminal surface of tissue-engineered tracheal graft was covered by a mature and highly-ciliated epithelium, whereas tracheal grafts without hTMSC sheets were covered by only a thin, immature epithelium. Therefore, hTMSC sheets on the luminal surface of a tissue-engineered tracheal graft can accelerate the tracheal epithelial regeneration, and the tissue-engineered tracheal graft with hTMSC sheets provides a useful clinical alternative for tracheal epithelial regeneration.