Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells

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• 作者：Hu Jingchao^a ; ^{hujingchao117@hotmail.com"" rel=""nofollow} ; Shu Rong ; ^a ; ^{shurongscience@gmail.com"" rel=""nofollow} ; Song Zhongchen^a ; Chen Lan^a
• 关键词：Amelogenin ; BMSCs ; Osteogenic ; DNA microarray
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2011
• 出版时间：13 May 2011
• 年：2011
• 卷：408
• 期：3
• 页码：437-441
• 全文大小：503 K

文摘

Extracts of enamel matrix proteins are used to regenerate periodontal tissues. Amelogenin, the most abundant enamel protein, plays an important role in the regeneration of these tissues. However, the molecular mechanisms by which amelogenin contributes to periodontal regeneration remain unknown. Using primary human bone marrow stroma cells (hBMSCs) transduced with lentivirus encoding human amelogenin (hAm), we performed genome-wide expression profiling to analyze the effects of hAm transduction on the regulation of genes involved in osteogenic differentiation. Our results revealed that BMP-2, BMP-6, OPN and VEGFC were up-regulated. These results suggest that hAm may be a key element in regulating hBMSCs osteogenic differentiation.