Expression and secretion of functional recombinant ¦ÌO-conotoxin MrVIB-His-tag in Escherichia coli

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• 作者：Bingmiao Gao ; Dongting Zhangsun ; Yuanyan Hu ; Yong Wu ; Lizi Sheng ; Licong Fang ; Xiaosa Wu ; Jinpeng Yu ; Sulan Luo
• 关键词：¦ÌO-conotoxin MrVIB ; Recombinant conotoxin ; Escherichia coli ; PelB leader ; Secretory expression ; Affinity chromatography
• 刊名：Toxicon
• 出版年：2013
• 出版时间：15 September, 2013
• 年：2013
• 卷：72
• 期：Complete
• 页码：81-89
• 全文大小：962 K

文摘

¦ÌO-conotoxin MrVIB is a 31-amino acid peptide containing three disulfide bonds isolated from the venom of Conus marmoreus, which is a selective antagonist of voltage-gated sodium channel (VGSC) Nav1.8 and has a long-lasting analgesic activity. Drug development of MrVIB has long been hindered over 15 years by difficult chemical synthesis and oxidative folding. Herein we describe a different approach based on the recombinant expression of gene MrVIB in Escherichia coli. A secretion vector pET22b(+)-MrVIB fused with pelB leader signal peptide and His-tag was constructed, which was transformed into BL21 (DE3) strain of E. coli. The recombinant conotoxin MrVIB-His-tag (rMrVIB-His) was successfully expressed and secreted into the periplasmic space of BL21 (DE3) cells. The pelB leader signal peptide was properly cleaved and three disulfide bonds were also formed properly to yield biological active rMrVIB-His. Folded rMrVIB-His in the periplasmic fraction was isolated with a Ni-NTA affinity column, which was further purified using reverse-phase high-performance liquid chromatography (RP-HPLC) and identified by liquid chromatography/mass spectrometry-ion trap-time of flight mass spectrometry (LC/MS-IT-TOF). Biological activity assay of rMrVIB-His showed it had good analgesic effects in three pain models.