Development of a novel terbium(III) chelate-based luminescent probe for highly sensitive time-resolved luminescence detection of hydroxyl radical

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• 作者：Guanfeng Cui^a ; Zhiqiang Ye ; ^a ; ^{zhiqiangye2001@yahoo.com.cn} ; Jinxue Chen^a ; Guilan Wang^a ; Jingli Yuan ; ^a ; ^{jingliyuan@yahoo.com.cn}
• 关键词：Terbium chelate ; Luminescent probe ; Hydroxyl radical ; Time-resolved luminescence ; Cell imaging
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 May 2011
• 年：2011
• 卷：84
• 期：3
• 页码：971-976
• 全文大小：564 K

文摘

Time-resolved (or time-gated) luminescence detection technique using lanthanide chelates as luminescent probes is a widely used and highly sensitive method for the biological applications. The developments of various functional lanthanide probes that can selectively recognize the biological targets are the essential objective of the technique. In this work, a unique Tb³⁺ chelate-based luminescent probe, N,N,N¹,N¹-[2,6-bis(3′-aminomethyl-1′-pyrazolyl)-4-(p-aminophenoxy)methylene-pyridine] tetrakis(acetate)-Tb³⁺(BMPTA-Tb³⁺), has been designed and synthesized for highly selective and sensitive time-resolved luminescence detection of one highly reactive oxygen species (ROS), hydroxyl radical (OH). The probe is almost non-luminescent, and can selectively react with hydroxyl radical to afford a highly luminescent Tb³⁺ chelate, N,N,N¹,N¹-[2,6-bis(3′-aminomethyl-1′-pyrazolyl)-4-hydroxymethyl-pyridine] tetrakis(acetate)-Tb³⁺ (BHTA-Tb³⁺), accompanied by a 49-fold increase in luminescence quantum yield with a long luminescence lifetime (2.76 ms). The luminescence response of the probe to hydroxyl radical is highly selective and insensitive to pH in the physiological pH range. For loading the probe into the living cells, the acetoxymethyl ester of BMPTA-Tb³⁺ was synthesized and used for the HeLa cell loading. Based on this probe, a background-free time-resolved luminescence imaging method for detecting hydroxyl radical in living cells was successfully established.