Optimization of a quantitative PCR based method for plasmid copy number determination in human cell lines
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- 作者：Lukas Fliedla ; 1 ; Florian Kasta ; 1 ; Johannes Grillaria ; b ; Matthias Wiesera ; Regina Grillari-Voglauera ; b ; regina.grillari@boku.ac.at" class="auth_mail" title="E-mail the corresponding author
- 刊名：New Biotechnology
- 出版年：2015
- 出版时间：25 December 2015
- 年：2015
- 卷：32
- 期：6
- 页码：716-719
- 全文大小：678 K
文摘
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Real time PCR based detection of plasmid copy number in human cells.
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Protocol including purification step.
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Decay of plasmid copy number in HEK293.
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Comparison of PCN in HEK cell lines and RPTEC/TERT1 and RS cell line.