Scube activity is necessary for Hedgehog signal transduction in vivo

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• 作者：Jacque-Lynne F.A. Johnson ; Thomas E. Hall ; Jennifer M. Dyson ; Carmen Sonntag ; Katie Ayers ; Silke Berger ; Philippe Gautier ; Christina Mitchell ; Georgina E. Hollway ; Peter D. Currie
• 关键词：Hedgehog signaling ; Zebrafish ; Muscle development
• 刊名：Developmental Biology
• 出版年：2012
• 出版时间：15 August, 2012
• 年：2012
• 卷：368
• 期：2
• 页码：193-202
• 全文大小：1606 K

文摘

The Hedgehog (HH) signaling pathway is a central regulator of embryonic development, controlling the pattern and proliferation of a wide variety of organs. Previous studies have implicated the secreted protein, Scube2, in HH signal transduction in the zebrafish embryo () although the nature of the molecular function of Scube2 in this process has remained undefined. This analysis has been compounded by the fact that removal of Scube2 activity in the zebrafish embryo leads to only subtle defects in HH signal transduction in vivo (). Here we present the discovery of two additional scube genes in zebrafish, scube1 and scube3, and demonstrate their roles in facilitating HH signal transduction. Knocking down the function of all three scube genes simultaneously phenocopies a complete loss of HH signal transduction in the embryo, revealing that Scube signaling is essential for HH signal transduction in vivo. We further define the molecular role of scube2 in HH signaling.