Super-resolution Microscopy in Plant Cell Imaging

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• 作者：George Komis¹ ; Olga &Scaron ; amajová ; ¹ ; Miroslav Ovečka¹ ; Jozef &Scaron ; amaj¹ ; ^{jozef.samaj@upol.cz" class="auth_mail" title="E-mail the corresponding author}
• 关键词：super-resolution microscopy ; structured-illumination microscopy ; photoactivation localization microscopy ; stimulated emission depletion microscopy ; stochastic optical reconstruction microscopy ; plant cell biology
• 刊名：Trends in Plant Science
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：20
• 期：12
• 页码：834-843
• 全文大小：1812 K

文摘

Although the development of super-resolution microscopy methods dates back to 1994, relevant applications in plant cell imaging only started to emerge in 2010. Since then, the principal super-resolution methods, including structured-illumination microscopy (SIM), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and stimulated emission depletion microscopy (STED), have been implemented in plant cell research. However, progress has been limited due to the challenging properties of plant material. Here we summarize the basic principles of existing super-resolution methods and provide examples of applications in plant science. The limitations imposed by the nature of plant material are reviewed and the potential for future applications in plant cell imaging is highlighted.