Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines

详细信息    查看全文

• 作者：Yajun Wu^a ; ^b ; ¹ ; ^{wuyajun0922@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Shuai Li^b ; ¹ ; ^{lishuaicl@sina.cn" class="auth_mail" title="E-mail the corresponding author} ; Haixia Li^b ; ^{lihaixiayes@163.com" class="auth_mail" title="E-mail the corresponding author} ; Chunzhi Zhao^c ; ^{zcz54dr@163.com" class="auth_mail" title="E-mail the corresponding author} ; Hao Ma^b ; ^{majordan@163.com" class="auth_mail" title="E-mail the corresponding author} ; Xiunan Zhao^b ; ^{xn_02080589@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Junhua Wu^b ; ^{wujunhua1971@163.com" class="auth_mail" title="E-mail the corresponding author} ; Kunlu Liu^b ; ^{liukunlu@126.com" class="auth_mail" title="E-mail the corresponding author} ; Junjie Shan^a ; ^b ; ^{shanjunjie001@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yuxia Wang^b ; ^{wangyuxia1962@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AAALAC ; Accreditation of Laboratory Animal Care International ; APCs ; antigen presenting cells ; CE ; capillary electrophoresis ; DCs ; dendritic cells ; ELISA ; enzyme linked immunosorbent assays ; FBS ; fetal bovine serum ; HBsAg ; hepatitis B virus surface antigen ; HPGPC ; high performance gel permeation chromatography ; iNOS ; NO synthase ; MPL ; 3-O-desacyl-4&prime ; -monophosphoryl lipid A ; NF-κB ; activating nuclear factor κB ; NK ; natural killer ; NO ; nitric oxide ; OD ; optical density ; TFA ; trifluoroacetic a
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：91
• 期：Complete
• 页码：248-257
• 全文大小：752 K

文摘

Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0 kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines.