Spanish Broom (Spartium junceum L.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: Preparation, characterization and antibacterial activity

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• 作者：Teresa Cerchiara^a ; ^{teresa.cerchiara2@unibo.it} ; Angela Abruzzo^a ; Rogers Alberto Ñ ; ahui Palomino^a ; Beatrice Vitali^a ; Renata De Rose^b ; Giuseppe Chidichimo^b ; Luca Ceseracciu^c ; Athanassia Athanassiou^c ; Bruno Saladini^d ; Francesco Dalena^b ; Federica Bigucci^a ; Barbara Luppi^a
• 关键词：Spanish Broom fibers ; Nanoparticles ; Chitosan ; Wound dressing ; Cellulose ; Vancomycin
• 刊名：European Journal of Pharmaceutical Sciences
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：99
• 期：Complete
• 页码：105-112
• 全文大小：640 K
• 卷排序：99

文摘

In this work, we propose as new wound dressing, the Spanish Broom fibers impregnated with vancomycin (VM) loaded chitosan nanoparticles. Spanish Broom fibers were extracted by patented method DiCoDe and the morphological, physical and mechanical properties were investigated. Chitosan nanoparticles were prepared by ionic gelation using different weight ratios between chitosan (CH) and tripolyphosphate (TPP). Nanoparticles were characterized in terms of size, zeta potential, yield, encapsulation efficiency, stability and drug release. Finally, the antibacterial activity against Staphylococcus aureus as well as in vitro cytotoxicity on HaCaT cells were evaluated.The best formulation CH/TPP 4:1 was selected based on the encapsulation efficiency and yield. Spanish Broom fibers impregnated with loaded nanoparticles showed an increased antibacterial activity against S. aureus compared to the same fibers containing VM without nanoparticles. Moreover, these fibers were not toxic to HaCaT keratinocytes cells. In conclusion, Spanish Broom fibers impregnated with VM loaded CH/TPP nanoparticles would appear to be a promising candidate for wound dressing application.