Mammosphere cells from high-passage MCF7 cell line show variable loss of tumorigenicity and radioresistance

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• 作者：Guozhu Xie^a ; ^b ; Junfang Zhan^a ; Yunhong Tian^a ; Ying Liu^a ; Zhixian Chen^a ; Chen Ren^a ; ^b ; Quanquan Sun^a ; Jianping Lian^a ; Longhua Chen^a ; Jian Ruan^a ; Changsheng Ye^c ; Aimin Sun^a ; Yawei Yuan^a ; ^{yuanyw66@yahoo.cn}
• 关键词：SLCs ; stem-like cancer cells ; BSLCs ; breast stem-like cancer cells ; MSs ; mammosphere cells ; ROS ; reactive oxygen species ; ATM ; ataxia telangiectasia mutated
• 刊名：Cancer Letters
• 出版年：2012
• 出版时间：March 2012
• 年：2012
• 卷：316
• 期：1
• 页码：53-61
• 全文大小：1366 K

文摘

Mammosphere culture of cancer cell lines is an important approach used for enrichment of stem-like cancer cells (SLCs), but over-subcultured cell lines have been experimentally shown to change properties over time. It remains unclear if mammosphere cells (MSs) derived from high-passage cancer cell lines retain the tumorigenicity and radioresistance seen in MSs from primary or low-passage cell lines. In this study, we report that mammospheres derived from MCF-7 sublines after different passage numbers were consistently enriched for CD44⁺/CD24^?low cells but were not consistently enriched for tumorigenic and radioresistant cells. The tumorigenicity and radioresistance of MSs were associated with their sphere-forming ability, proliferation ability in vitro, and intracellular reactive oxygen species (ROS) levels. The radioresistant MSs showed significant cell cycle arrest in G2/M phase after X-ray irradiation and expressed higher ataxia telangiectasia mutated (ATM) mRNA levels. These results suggest that MSs from high-passage cancer cell lines were not consistently enriched for stem-like cancer cells with higher tumorigenicity and enhanced radioresistance.